ChazR Posted March 16, 2009 Posted March 16, 2009 We're planning on expressing a sequence that contains an insertion of two copies of a gene in a tandem repeat. Should we be concerned with recombination or 'slippage' and loss of a copy during cloning and expression in XL2-Blue/BL21 DE3 E. coli systems? We have the option of using it from another organism, but it would be a eukaryote and we are concerned about codon optimization and poor expression.
Recommended Posts
Create an account or sign in to comment
You need to be a member in order to leave a comment
Create an account
Sign up for a new account in our community. It's easy!
Register a new accountSign in
Already have an account? Sign in here.
Sign In Now