is there hope for "goopy" cell culture media?

[jobradfield]

I'm trying to see if lubes with glycerol contribute to yeast infections and I need to design a media that will mimic the conditions inside the vagina.

 

*It needs to be carbohydrate-poor enough that I can create a (-) control without being too unlike vaginal fluid.

 

*The lubes have to dissolve into it (water-based will work fine, but I don't know what to do about silicone or oil-based lubes)

 

*It should have a chemical composition as similar as possible to vaginal fluid.

 

I have enough information to create rudimentary synthetic vaginal fluid for the media, but I expect it to be "goopy". I've only ever done cell cultures in agar or liquid media and I don't know if it will work in the fake-vaginal fluid goo.

 

any suggestions?

 

also I saw a pending patent on a synthetic cervical mucus, has anyone seen it before?

is it fore sale anywhere?

[jake.com]

I'm trying to see if lubes with glycerol contribute to yeast infections and I need to design a media that will mimic the conditions inside the vagina.

 

*It needs to be carbohydrate-poor enough that I can create a (-) control without being too unlike vaginal fluid.

 

*The lubes have to dissolve into it (water-based will work fine, but I don't know what to do about silicone or oil-based lubes)

 

*It should have a chemical composition as similar as possible to vaginal fluid.

 

I have enough information to create rudimentary synthetic vaginal fluid for the media, but I expect it to be "goopy". I've only ever done cell cultures in agar or liquid media and I don't know if it will work in the fake-vaginal fluid goo.

 

any suggestions?

 

also I saw a pending patent on a synthetic cervical mucus, has anyone seen it before?

is it fore sale anywhere?

 

You mean this stuff?

 

Composition for synthetic cervical mucus formulation Document Type and Number:United States Patent 6953775

Abstract:The present invention is a composition for a synthetic mucus formulation with viscosity, spinnbarkeit, and pH comparable to that reported for human cervical mucus.

 

On another site it said this too

 

A synthetic formulation has been developed with viscosity, spinnbarkeit, and pH comparable to that reported for human cervical mucus. The formulation contains guar gum crosslinked with borate ion, mucin (dried porcine gastric), and a mixed preservative system in pH 7.4, 0.1M phosphate buffer. The guar gum source, mucin concentration, and method of preparation were shown to be critical factors in the performance of the formulation.

 

 

If you do, it's not available yet.