Need Help - Stumped By Immunology Prof !!

[Tiger1]

This is almost an outright plea for spoonfeeding, albeit not before 8 hours of study hasn't produced much of a clue!! Here's the question:

 

"Using various inbred strains of mice, design an experiment to determine the importance of the three classes of MHC antigens in graft rejection. Include results and interpretation of the results in your answer"

 

I've got a good grip on the 3 types of MHC antigens and their relative importance in rejection. Cl II being most implicated (HLA-DR) followed by Cl I (HLA-B>A>C) and trailed by Cl III which I probably need to tie involvement of complement to and the fact that Cl III is usually expressed on endothelial cells. My problem is the damned mice. Any ideas or is more info. needed?

[ecoli]

how can you interpret the results of a hypothetical experiment?

[Tiger1]

I guess the advantage of being able to design the experiment is I can take the liberty of having any results I want to as long as they support in some way the question being asked. Do you have any thoughts on that Mr. gut bacteria or are you just a terminal smart ass.

[ecoli]

Do you have any thoughts on that Mr. gut bacteria or are you just a terminal smart ass.

well my smart ass-ery hasn't ended yet, so I wouldn't call it "terminal"

[Tiger1]

Well you got me there, I was just hoping you had some input on my homework dilemma (no psuedohip avatar).

[jimmydasaint]

Some info here man. You need to get back to basics then build on it by looking at 'Materials and Methods' sections of papers and then look at the techniques for mice that are genetically homogeneous for any given MHC serotype.

 

I would look here for basic theory:

http://pathmicro.med.sc.edu/ghaffar/mhc2000.htm

 

Then look at the M & M section of this type of paper for practical techniques

Paper

 

Hope these help