Jen_23 Posted June 18, 2009 Posted June 18, 2009 Hi All, does anyone have a protocol which they have used to successfully transform a plasmid into a staph species? I've tried 4 protocols in duplicate on 8 strains and I'm getting nowhere!! Thanks Jen
CharonY Posted June 18, 2009 Posted June 18, 2009 Were the protocols you used specifically for S. aureus? AFAIK there are a few out there. If those do not work I would start troubleshooting the process than yet try another one (doing it in duplicate is not really troubleshooting, but rather counts as doing a backup).
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