Carbamoyl phosphate?

[John Ferdinand]

What are the real components of carbamoyl phosphate?

 

When studying for my BC exam, specifically pyrimidine biosynthesis, I had some difficulty tracing back the origin of the various atoms in the pyrimidic ring. Numbering from 1 to 6, starting "down" and running clockwise, I realized atoms 1, 4, 5 and 6 (respectively 1 nitrogen and 3 carbons) came from aspartate but the two leftstanding (C2 and N3) were not that easy. Some sources say C2 comes from CO2, others from HCO3-, which one is it? Is CO2 transformed into HCO3- before the reaction? And is it true that, in fact, Carbamoyl phosphate is made from HCO3- and the glutamine's amide?

 

Thanks in advance

 

John

[GDG]

What are the real components of carbamoyl phosphate?

 

When studying for my BC exam, specifically pyrimidine biosynthesis, I had some difficulty tracing back the origin of the various atoms in the pyrimidic ring. Numbering from 1 to 6, starting "down" and running clockwise, I realized atoms 1, 4, 5 and 6 (respectively 1 nitrogen and 3 carbons) came from aspartate but the two leftstanding (C2 and N3) were not that easy. Some sources say C2 comes from CO2, others from HCO3-, which one is it? Is CO2 transformed into HCO3- before the reaction? And is it true that, in fact, Carbamoyl phosphate is made from HCO3- and the glutamine's amide?

 

Thanks in advance

 

John

 

The chart in Wikipedia has the atoms color-coded, and easy to trace.

 

CO2 forms HCO3- when it dissolves in water, so they are essentially the same thing as far as biochemistry goes.