Sulfation in cells...What gets sulfated?

[lciaquin]

I added heparanase to cleave sulfated GAG chains or add chlorate to inhibit sulfation and still have radioactively labeled sulfates getting incorporated. What could it be getting incorporated into? Thanks!

[CharonY]

It depends a bit whether you let the cell metabolize the acquired sulfate. There are a rather largish amount of molecules into which sulfur gets incorporated as e.g. amino acids or iron-sulfur clusters.

Edited July 6, 2009 by CharonY

[Theophrastus]

Wait, I'm actually rather confused. Are you refering to an isolated experiment with heparan, or is it a cellular process you're working with? A little more articulation would be most appreciated.