Efraim Racker Vs Peter Mitchell

[Jeju]

In the most accepted hypothesis of oxidative phosphorylation, P . Mitchel gave an account on d transport of electrons in d electron transport chain on d inner membrane of d mitochondria , d generation of electrochemical gradient that drives d synthesis of ATP, the presence of d enzyme ATPase (a membrane bound protein in the inner membrane of the mitochondria) and hence the generation of ATP.

In Rackers experiment, the membrane was distorted to isolate d fragments of d membrane from d protein which was d F1 Fo ATPase. On his conclusion, electron transport was possible on d fragments, witout phosphorylation, and there was phosphorylation by d protein fraction and then,d recontruction gives oxidative phosphorylation. My question is this:

- Where was d energy derived from in Rackers experiment that caused d phosphorylation of ADP since d membrane is damaged? Cos an electrochemical gradient cannot be generated in d fragmented mitochondria.

[John Cuthber]

What's with all the "d"s

[Jeju]

I'm sorry if i had caused any confusion, i somtimes use "d" to simply mean "the". Please, any body with an idea should help , thanks.

[Sau1us]

Well, one important factor not mentioned was the quantity. I wouldn't be surprised if ATP synthase could operate without a H+ gradient, but I would be if they operated at the same level. Also, the ATP generated in the fragment case ATP may be immediately cleaved to at least keep the reaction going. Without knowing more I can only speculate.

[Jeju]

With this hypothesis of oxidative phosphorylation, the study for me has been too theorytical rather than being experimental. I seems to agree with you, but do you have any report to guide your speculations? Please i will be so delighted to understand this, and thanks 4 your contribution.

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Consecutive posts merged

I'm wondering why the low response. Pls share with me whatever your opinion, thanks all.

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Merged post follows:

Consecutive posts merged

I'm wondering why the low response. Pls share with me whatever your opinion, thanks all.