Small unilamellar vesicles + Dehydrogenase constructs

[dafenris]

Hi guys

 

I'm really stuck right now and I hope someone can help me. I just began my master thesis and I have to prepare an experiment. We would like to produce small unilamellar vesicles (SUVs) and insert a dehydrogenase (retinol dehydrogenase, RDH5) into them, since its a membrane protein. Could anyone tell me, how this could be done? I know how to prepare SUVs (read some papers about this procedure) but I don't really know, how to insert the dehydrogenase constructs ?! Perhaps express them in E.coli first and then mix them somehow with the SUVs?

 

I hope someone can help me

 

Cheers,

 

dafenris