How to calculate Km and Vmax without using a graph?!!

[mark83]

ok, the question is:

Calculate Km and Vmax for the enzyme reaction with data as below WITHOUT using a graphical method. (10 marks).

So all the data they have given is a 2 column table, time values that increase from 0 to 4.89(like 0, 0.052449965, 0.104970974, 0.157565146..etc) and the other column has concentration values that decreases from 70 to 1 (like 70, 69, 68, 67, 66..etc). i beleive this is the substrate conc. but i hav no idea, they wont tell me the concentration of what it is..maybe substrate, i have no idea.

 

so how do i calculate Km and Vmax for this without using a graphical method. what equations shud i use? at least give me the answer ill try to work it out somehow.

 

CHEERS!

[CaptainPanic]

If you already know how the graphical method works, you should be able to derive the formulas from such a graph. (Like a straight line with slope "a", not through the origin (0,0) will give something like Y = a*X + b).

 

There is no alternative way - the graphical method and formulas are essentially the same method.

 

Of course it's easier if you don't have to derive a formula - which is why I will give you the following: I'm not 100% sure that we're talking about the same thing but I have a strong feeling that you're dealing with "Michaelis Menten" kinetics here. That's a keyword that should give you some Google hits.

 

sorry for not answering your question - it's our forum policy not to answer homework questions.