Enzyme kinetics

[Aisha_18]

hi, I can really use some help. How would you convert ethanol substrate from 50 microlitres to micromoles/ unit? i dont know if this will help but the ethanol substrate was made as follows

1 mL of ethanol (95%v/v) was diluted with 199 mL of distilled water making the final concentration of the stock 100mM. 15 mL of this stock was thenplaced with 85mL of a buffer solution to make the final concentration 15mM. This stock was used for the enzyme assays.

[ggsteam]

Dear Aisha,

 

I am pretty confused about your calculations (I mean the stock concentration of 100mM), I am not sure where did you get that from:)?

 

You need to give us a little bit more information about the assay and about your conversion. I do not understand how you want to convert 50microliters (which is a volume unit) to 50micromoles (which is a concentration unit). Please give us more informations about it:)

 

GGSTEAM

 

Ps. If you have a problems with converting the units sometime ago I came up with creazy idea how to deal with it:) Have a look here http://bio-ggs.blogspot.com/2009/11/scientific-prefixes-calculations.html