hi, I can really use some help. How would you convert ethanol substrate from 50 microlitres to micromoles/ unit? i dont know if this will help but the ethanol substrate was made as follows

1 mL of ethanol (95%v/v) was diluted with 199 mL of distilled water making the final concentration of the stock 100mM. 15 mL of this stock was thenplaced with 85mL of a buffer solution to make the final concentration 15mM. This stock was used for the enzyme assays.

Given that,

 

Moles = Mass / Molecular mass

 

And

 

Density = Mass / Volume

 

Density = (Moles x Molecular mass) / Volume

 

Therefore,

 

Moles = (density x volume) / Molecular mass

 

Once you have the number of moles of ethanol, you should be able to convert that into concentration easy enough

Thanks,but i am not sure what volume to use given my the dilutions. Do i just use the 1ml for the ethanol? Or just use whatever volume of the ethanol substrate i would have used.

you are to use 1mm of the ethanol.