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Biochemistry - Length of peptide chain

Aisha_18
in Biochemistry and Molecular Biology

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How would you detemine the length of an amino acid chain from a formalin titration of a hydrolyzed an unhydrolyzed peptide. My ratio of carboxylic groups came out to be 2.5, but how do i determine the chanin length givien this information?

Ok, so you are aware what you actually measure with this method (carboxylic groups). How many free carboxylic group does e.g. an intact dipeptide possess. How many if it is hydrolyzed?

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