2D gel analysis of eukaryotic ribosomal proteins

[Guest alirman]

Hello,

Would anyone know of a research performed on eukaryotic ribosomal proteins (preferably S. cerevisiae) using 2D gel electrophoresis in association with mass spectrometry.

Your help is greatly appreciated

[LucidDreamer]

Wow, tough question. Its not likely that anyone on this forum (even as smart as some of them are) would know that information off hand, but i could be wrong. For that kind of information I usually use PubMed: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi

or scifinder, which can be found in most university libraries. Of course, if you’re asking this question there is a good chance you already familiar with both of these things. I would also suggest using Boolean operators liberally to save time. Just for kicks I ran a search in pubmed and came up with this link: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=10874046

<--- It has to do with chloroplast ribosomes and not yeast, sorry. But, the researchers apparently used both electrophoresis and mass spectrometry so maybe it might help. Good luck.

[admiral_ju00]

If you don't get an answer here, I bet you that someone here will(or already does)

http://micro.nwfsc.noaa.gov/forums/

[Sayonara]

Hello' date='

Would anyone know of a research performed on eukaryotic ribosomal proteins (preferably S. cerevisiae) using 2D gel electrophoresis in association with mass spectrometry.

Your help is greatly appreciated[/quote']

Are you talking about specific research, or any involving the above?

[Sayonara]

Wow, tough question. Its not likely that anyone on this forum (even as smart as some of them are) would know that information off hand, but i could be wrong.

Do you think you could avoid doing that?

[badchad]

Using pubmed, you can find numerous studies. If your looking for info about the actual technique of 2d Gel electrophoresis google it. Basically, it can separate a protein using two dimensions (hence the name). One of the dimensions is size, or weight, the second is by charge. You could look for a change in a protein as a result of a condition and then make an inference about it. I think you may be able to separate many proteins simultaneously, so you could for instance try and identify a set of proteins that are upregulated during S. cerevisiae replication, or something and target those for a therapeutic target. I've read a couple papers on it, but that was a while ago......pubmed should have plenty

[daisy]

You need to do a pub-med search on the NCBI site.

[CharonY]

You could look for a change in a protein as a result of a condition and then make an inference about it.

 

I suppose you mean a change in protein abundancy? That's theoretically possible but for a true quantification you got to use techniques like radioactive labelling or DIGE, or the usage of heavy nitrogen.

With 2D PAGES as compared to 1D you get a better resolution and therefore you can visulize more proteins.

And in fact there are plenty of papers regarding the proteome of fungi.

I don't think that there are 2d gels only woth ribosomal proteins around though, since one of the strength of this technique is the visualisation of a lot of proteins in one gel. If one could separate the ribosmal proteins from all others (at the moment i'd have no clue how short of His-tagging and purifying them) an 1D PAGE should suffice.