Transcription and RNA production?

[proberson88]

Here's the question:

 

If chromatin is digested with an endonuclease to produce 200-bp fragments, and these fragments are then used to transcription experiments, very little RNA is made. Provide an explanation for this observation.

 

I am not sure what they are looking for here. It seems that less RNA would be made because RNA is complementary to DNA and if the DNA is shorter then naturally the RNA would also be. But I am not sure what else could be the cause.

[dttom]

You may consider the processes in RNA transcription. What is required in the process, and whether such a factor or factors is (are) removed by fragmentation of DNA?

[CharonY]

And sometimes answers can be found upstream.