extracting ascorbic acid (vitamin c) out of ginger?

[Abchirk]

Hey I have searched in the internet, but I didn't find anything related to that. I found how to extract the hot substances like gingerol.

But I didn't find anything for ascorbic acid. So does anyone has an idea? I want to quantify the amount of vitamin C in ginger. It is for a project work at school.

 

Any suggestion is welcome!

[Horza2002]

Seeing as Vit C is an acid, you should be able to rextracted it by washing with an basic solution followed by reacidifying it

[Abchirk]

Yeah and then maybe titration, but we have to use modern analytic methods like HPLC etc.

 

I found some papers...

Does anyone has free access to papers like this:

- quantifying via GC - it would be very appreciated.

I already got this one:

- quantifying via HPLC -, but I guess our HPLC doesn't have such column.

 

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Consecutive posts merged

hm well, I got all papers now...

 

I have decided to use HPLC with a gradient (oH-level ~2)

Because you need something which prevents the ascorbic acid of degrading to dehydroascorbic acid. You can achieve this with substances which block the oxidizing of Ascorbicacid or a acid like metaphyophoric acid which also safes the ascorbic acid.

 

[John Cuthber]

Since vitamin C is very soluble in water there's nothing much to gain by using a base to extract it. Also, it is less likely to be oxidised by the air if it is in neutral or acid solution.

(BTW, a lot of the column manufacturers supply "application notes" for their columns. It's blatant advertising, but it's a good way to find out about analytical methods, on-line, for free.)

[Abchirk]

(BTW, a lot of the column manufacturers supply "application notes" for their columns. It's blatant advertising, but it's a good way to find out about analytical methods, on-line, for free.)

 

Yes, I know I was a bit in hurry...

But I asked my teacher now and he said that I could do it with pH=2 also on the column, but I have to re-neutralize the column every day...

 

Thanks for your help!