catalase non kit assay

[veronika]

Oh yes, Im having a hell of a time trying to find in detail recipees, protocols or whatever you want to call them for the catalase hydrogenperoxide assay. And not the kit things.If someone has the Beutler method recipee, well, yoopieeee!! And I mean real details , the composition and molarity of the buffer and the reaction mixture, the volumes , everythin. Oh and please dont forget the calculation formula in the end! Yeah, this is just as it looks like- HEEEELP!

[CharonY]

There are dozens of different catalase assays. You need to put it into a little context. Otherwise take a look at some reviews. One of the oldest is probably

The assay of catalases and peroxidases.

MAEHLY AC, CHANCE B.

Methods Biochem Anal. 1954;1:357-424.

[veronika]

There are dozens of different catalase assays. You need to put it into a little context. Otherwise take a look at some reviews. One of the oldest is probably

The assay of catalases and peroxidases.

MAEHLY AC, CHANCE B.

Methods Biochem Anal. 1954;1:357-424.

 

Sorry CharonY, I was so desperate last night that I didnt explain my position in full. You see, Ive been digging a couple of hours on pubmed, highwire, hinari and I actually SAW ALLTHOSE BEAUTIFULL ARTICLES WITH direct approach to the more than helpful protocols. But .... there is one little problem. None of them is full text free of payment.And being a molecular biologist in a country that doesnt have money for science nor scientists it really means a lot.Yeah, I know theres dozens...sigh. If I had been able to take up any of them or better even, several of them,full text online without paying 15, 20 or even 35 dollars per article, I wouldnt trouble people on the forum. Truth be said I stumbled over two or three recipees and I downloaded them.See what I can do. But I had a workbook with the slightly modified Beutler method for measuring catalase activity spectrophotometrically on 230 nm wavelength based on the degradation of hydrogene peroxide and it was such an elegantly simple method. The problem is my workbook has vanished from my room so as you see Im on the beginning. And the main stumblesotne is that I cant remember the buffer used in that protocol. Was it potassium phosphate or Tris chloride and what was the molarity and PH... Ill try and see the optimum Ph for rat catalase activity and then Ill know which buffer to use according to the optimal Pk for each of these solutions. Anyway thank you and I hope we will hear again!!

[JGK]

Are these of any use?

 

From Sigma Aldrich.com

 

From oxis.com

[CharonY]

Ah, photometric assay in liquid. I think the one from Sigma is an assay often done in courses. Regarding free papers, if your access is limited you may want to search open access journals first (e.g. BMC, PloS, Biomed central). Just a thought.

[veronika]

Ah, photometric assay in liquid. I think the one from Sigma is an assay often done in courses. Regarding free papers, if your access is limited you may want to search open access journals first (e.g. BMC, PloS, Biomed central). Just a thought.

 

Yup, that sort of thing.Actually I have some tissue homogenates (as years before) that I want to assay for catalase activity. And I ll check a bit more thoroughly the open access journals and see what I can dig out. Though the sigma aldrich mini manual for catalase isnt bad!!

Well, see you!

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Are these of any use?

 

From Sigma Aldrich.com

 

From oxis.com

 

yes, thank you and I think I came up on the sigma protocol myself too and it looks like sufficient for what I need. the other one isnt bad too so Ill keep them handy.Thanks for taking the time!!