curtis123 Posted February 25, 2010 Posted February 25, 2010 I am having a hard time understanding how to interpret electrophoresis gels. I have spent a lot of time looking into this and need some help. Why do double digests sometime create one fragment on the gel? I thought that there should be more fragments since the DNA is being cut twice?!
CharonY Posted February 25, 2010 Posted February 25, 2010 It depends on a) the form of the DNA, b)how many restriction sites for the given enzyme are present on the DNA molecule c) where the restrictions sites are and d) the resolution of the gel (i.e. which sizes are finally visible).
Recommended Posts
Create an account or sign in to comment
You need to be a member in order to leave a comment
Create an account
Sign up for a new account in our community. It's easy!
Register a new accountSign in
Already have an account? Sign in here.
Sign In Now