sci-nuke Posted February 28, 2010 Posted February 28, 2010 In Golden rice three genetic traits are recombinant in its dna: Phytoene synthase (psy), phytoene desaturase (crt1) and lycopene cyclase (lyc). I cannot find where lycopene cyclase is derived from which organism (in this case golden rice)
CyanideScience Posted March 7, 2010 Posted March 7, 2010 As far as I know, and I'm no expert, lycopene cyclase is produced in the endosperm of wild-type rice and was only thought to be needed until further research revealed it was unnecessary, since it was already being produced.
DctrZaius Posted October 13, 2010 Posted October 13, 2010 I'm pretty confident that all three genes are taken from daffodils.
KaH4 Posted June 16, 2011 Posted June 16, 2011 For any future people writing about this. Phytoene synthase (psy) was taken from daffodils but is now taken from maize for Golden Rice 2. This produces 23 times more carotenoids. Phytoene desaturase (crt1) is taken from the bacterium Erwina Uredovora. I believe CyanideScience is right that lycopene cyclase (lyc) is already present. It mentions that they thought they needed to put it in originally but found this was not necessary on the Golden Rice website! Very complicated website but one you can guarantee to be right as many site say conflicting things! I think that the rDNA is then placed into agrobacteria. The agrobacteria is then placed in a petri dish with rice embryo. The agrobacteria will transfer rDNA to Rice DNA. Thats what I have understood from research anyway.
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