Graphs !! Carbs and enzymatic hydrolysis

[jack-1-richards]

Hey guys,

 

I'm a new member - In my first year of biomedical science.

 

My Biochemistry module consists of 50% examination (human metabolism)

And 50% consists of practical write ups.

 

I'm confused about one graph that is required, the practical in question consists of "estimation of reducing carbohydrates" and "enzymatic and acid hydrolysis of glycogen"

 

Values aside.. I need to plot the following graph on the latest version of excel:

 

Standard curve of concentration and absorbance - done this with no problems.

On the SAME graph, I must also plot:

Absorbance against time,

Glucose equivalents against time,

%Hydrolysis against time.

 

Anyone have any ideas how to go about this?

I could plot a third axis for time, but what do i do about glucose equivalents and %hydrolysis?

 

Really appreciate any help you could provide.

I have tried emailing my tutors, but it is Easter break so they aren't picking up.

 

This is probably a very easy task, but I've thought into this so much that I cant think clearly anymore !

 

Thanks again,

Jack.

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Merged post follows:

Consecutive posts merged

I've worked it out, thanks anyway.

 

Now a beast of a discussion to do about estimating fatty acid chain length from saponification value. Gotta be no more than 30 carbons long yeah?