Type of stimuli for BEAS2B and A549 cell lines?

[Lieon]

Hi, i'm new to this forum. I'm currently undertaking an honours research programme at my university. I'm running an experiment to measure interleukin-8 release from bronchial epithelial cell lines (BEAS2B & A549) via ELISA, and was wondering what kinds of stimuli would be appropriate? So far the stimuli i'll be using are: interleukin 1, LPS, TNF alpha, and a PAR agonist SLIGRL.

 

I have plated the cells on a 24 well plate and will be applying the stimuli to them in two days. Any input would certainly be helpful. Cheers.