Genecks Posted April 22, 2010 Share Posted April 22, 2010 I'm looking at the action of the Sau3A enzyme. What exactly is it cutting? 5'--- What does that third hyphen represent? Picture attached. Link to comment Share on other sites More sharing options...
Greippi Posted April 22, 2010 Share Posted April 22, 2010 (edited) It doesn't matter what the third hyphon it is. I ASSUME it could be any base and that the sequence it needs for recognition is just GATC. However, I'll just check that and get back to you in a second. Just looked it up on NEB cutter and that's correct. Edited April 22, 2010 by Greippi 1 Link to comment Share on other sites More sharing options...
CharonY Posted April 22, 2010 Share Posted April 22, 2010 The hyphens just indicate that the DNA sequences continues from there.... Link to comment Share on other sites More sharing options...
Mr Skeptic Posted April 22, 2010 Share Posted April 22, 2010 5'---GATC--- 3'---CTAG--- it gets cut as shown. The hyphens are just placeholders. Link to comment Share on other sites More sharing options...
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