what's wrong with my RNA samples

[fengduhua]

1 to 4 are arabidopsis leaf ,5 to 8 are arabidopsis root

why there are so many bands in leaf tissue,and what is the band in front of 28s rRNA

[Greippi]

Eeek, your picture isn't showing up.

[CharonY]

Depending on your isolation additional bands are to be expected, especially in very active tissues. For quality control one usually would check the 28/18s ratio. Very high MW bands can also be co-isolated DNA.

[fengduhua]

Depending on your isolation additional bands are to be expected, especially in very active tissues. For quality control one usually would check the 28/18s ratio. Very high MW bands can also be co-isolated DNA.

 

thanks for your reply, does it have such a possibility that the chlorophy body rRNA and chondriosome rRNA are extracted from leaf tissue meanwhile

[CharonY]

It depends precisely on what you are seeing and your extraction protocol. Are the additional bands on the height of the 16S rRNA?