Yeast 2 Hybrid!

[CoolScience]

I need help with my yeast 2 hybrid screen:( Can I get many false positive (blue) due to my own reagents?

I am looking for interactions between several genes. I have started by checking whether my Yeast strain auto-activated. And surprisingly it does! I have bought and tested few strains and all auto-activate! I am now questioning my X-Gal, LacZ, and filter/nitrocellulos papers! Can I get soooo many false positive because of my reagents, or it is true that my yeast auto-activate on their own?

[dttom]

your yeast should be autoactive to express BD-X, so when you fused your yeast with AD-Y plasmid can they interact (potentially) to activate (if they do interact) a third set of reporter.