CoolScience Posted June 13, 2010 Share Posted June 13, 2010 I need help with my yeast 2 hybrid screen:( Can I get many false positive (blue) due to my own reagents? I am looking for interactions between several genes. I have started by checking whether my Yeast strain auto-activated. And surprisingly it does! I have bought and tested few strains and all auto-activate! I am now questioning my X-Gal, LacZ, and filter/nitrocellulos papers! Can I get soooo many false positive because of my reagents, or it is true that my yeast auto-activate on their own? Link to comment Share on other sites More sharing options...
dttom Posted June 15, 2010 Share Posted June 15, 2010 your yeast should be autoactive to express BD-X, so when you fused your yeast with AD-Y plasmid can they interact (potentially) to activate (if they do interact) a third set of reporter. Link to comment Share on other sites More sharing options...
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