I need to create a new gene construct.

[J.S.P.O.]

Hey everyone, I am in my first year of graduate studies and I have a great idea for a gene construct. I know some basics about plasmids and restriction digest I just don't know where to start. So how do I go about creating this idea of a gene construct I have?

[CharonY]

That is incredibly vague and depends a lot of the nature and use of the construct. To answer in an equally vague fashion:

 

First step is often PCR (or several PCR steps for specific constructs) followed by cloning.

[J.S.P.O.]

I do understand that this is vague. What I want to know is where can I find a good source of information on molecular cloning techniques.

[CharonY]

Get the book "Molecular cloning" from Sambrook.