biosyn12 Posted July 22, 2010 Posted July 22, 2010 (edited) Synthetic peptides could be used for the following purposes: • To verify the structure of naturally occurring peptides • To study the relationship between structure and activity of biologically active proteins and peptides and establish their molecular mechanisms and • To develop new peptide-based immunogens, antibodies, hormones, vaccines, etc. Synthetic peptides may range from 2 to 120 amino acids. Small synthetic peptides (in general less than 10 amino acids in length) have the advantage of rarely inducing antibody formation and can be tailored to move across blood brain barrier. Peptides for antibody production are generally of 15 to 25 amino acids. Such peptides can be conjugated to a protein carrier such as KLH, BSA etc. which can induce antibody production and used for both in vitro and in vivo assays. Edited July 22, 2010 by biosyn12 1
jimmydasaint Posted July 22, 2010 Posted July 22, 2010 (edited) What is the minimum size of peptide that can induce an immune response and why does it induce the response? Moreover, what type of polyclonal antibodies are produced? Moreover, does the minimum antigen size also apply to monoclonal antibodies? Can you please be specific and cite references. Thank you. Edited July 22, 2010 by jimmydasaint
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