Streptomyces Globisporus

[apricimo]

Hello,

 

 

I am working with the mentioned species and I am trying to isolate the genomic DNA. This species makes spores after some time but overall spores form throughout the growth period. Does anyone know a good strategy for isolating genomic DNA from spores?

 

Or have experience with working with sporulating streptomyces and isolating their DNA?

 

Thank you.

[Horza2002]

I work with S. coelicolour, however, I tend to extract the mycelia to isolate the natural product im studying. Are you attempting to isolate the DNA from the bacteria or from a spore?

[apricimo]

I work with S. coelicolour, however, I tend to extract the mycelia to isolate the natural product im studying. Are you attempting to isolate the DNA from the bacteria or from a spore?

 

Ideally from mycelia but it seems that spores form throughout the growth. Maybe my prep sucks. I've isolated the gemomic DNA before, but have had a hard time since. I use a bactozol kit it works nicely if there are no spores.

 

What medium do you use for your growth to get your species to produce natural products?

[Horza2002]

I usually use LB media in a liquid state...I then place a sterlised permeable membrane over this...then add the spores onto of that. Let them grow for three days. After that I lift the membrane off which normally leaves the mycelia left which I then extract for the natural product. I don't work anything with the DNA so I don't know if theres anything wrong with your prep.