Gamewizard Posted March 20, 2011 Posted March 20, 2011 Hi, I need some help on this type of question. How would I calculate this ? If the absorbance at 540 nm of a sample of blood diluted 201 times in Drabkin's solution is 0.511 and that of a 0.572 g/L haemoglobin in Drabkin's solution is 0.325, what is the haemoglobin concentration of the blood sample in g/L? (the answer should be to one decimal place) I tried to work this out twice using the hb concentration formula which is absorbance of test*concentration of ref. prep. *dilution of blood/absorbance of ref. prep. but the answers I got was incorrect, and I cant tell where i am going wrong so can someone please have a look at this. Thanks
Schrödinger's hat Posted March 20, 2011 Posted March 20, 2011 Post your working, makes it easiest to see where you went wrong. You can use [math]\text{[math]}[/math]and[/math] to enclose equations to make it a little easier to read, also \frac{upperstuff}{lowerstuff} will make a fraction
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