Enzyme catalysis

[Stefan-CoA]

Hi all

 

I'm stuck with a question concerning the conversion of p-nitrophenol to hydroquinone and nitrite. It's an enzyme catalysed reaction that uses an O₂ equivalent and 2 NADH equivalents. I need to determine which co-factor is involved as well as the exact mechanism of action. I got as far as determining FAD/FMN as the co-factor (I hope), but beyond that I'm stumped. A gentle hint would make me a very happy boy.

 

Thanks in advance

 

S

[sikander]

In this reaction oxidative release takes place of p-nitrophenol to hydroquinone through a bacterial enzyme .Thats the only hint i can give you , you can proceed further .

[Stefan-CoA]

Thanks

 

But we managed to figure it out. For interest's sake; it involved an FAD-peroxo intermediate as well as mono-oxygenation and resonance about the benzene ring