Origin of Replication Compatability

[apricimo]

Looking to coexpress two vectors pCDFDuet and pETDuet in E. coli. Origins of replication are CDF and F1 respectively. Anyone know if these are compatible?

 

Yes they are compatible

[CharonY]

IIRC the duet vectors are routinely used for multiple co-expression, so as long as you use a host strain that is compatible to both plasmids, you shold be fine. Novagen also sold at least one of those. Novablue or something like that. Other strains include B834 and/or Bl21 derivatives, I think. But better check that out, as my recollection is a bit hazy on that.