Flow cytometry

[Amela]

Hello there,

I wonder if the FIX/PERM solution used during the Intra Cellular staining Could remove or kill red blood cells that could be present in the sample???

Thank you

[Bobr]

I cannot see the reason why it should, but I may be wrong. In that case, I would like to know why

[Amela]

The Permeabilisation solution that allows the antibodies to enter the cells could affect the integrity of the membrane and thus RBC may die cos of that, I was just wondering if the fixation solution could prevent this??

Some of my colleagues in the lab noticed that after ICS experiment RBC are reduced.....

 

 

 

 

 

 

 

[CharonY]

Basically all permeabilization and fixation methods put stress on cells. The composition of the solution and the resilience of the cells varies somewhat, though.

Edited April 19, 2011 by CharonY

[Amela]

Ok, Thank you, Then any tips to get rid of the RBC that persist in the PBMC solution after Blood separation with Ficoll gradient???