Farnesylated proteins on a western blot. urgent!

[futureless]

Hi, does anyone know if farnesylation could affect the mobility of a protein in a western blot? In particular, could farnesylated and unfarnesylated forn a doublet for a certain protein? or does farnesylation not show up, and the two forms would just migrate the same distance? help is very much appreciated, thanks in advance

[CharonY]

Well depends on the resolution and type of the gel as well as size of the protein. If you are in a very well resolved range it could be possible to see a shift.