beths Posted June 14, 2011 Share Posted June 14, 2011 Hi All, I'm using flow to measure the size of my cells. I split the cells and get a pellet and resuspend them in a bath solution (140mM NaCl, 2.5mM KCl, 1.2mM CaCl2, 0.5mM MgCl2, 5mM Glucose and 10mM HEPES). However, when I measure the cells in Hypotonic solution (same as before but 75mM NaCl), the cells seem to be decreasing in size rather than increasing. Any ideas? Link to comment Share on other sites More sharing options...
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