wonder Posted October 13, 2004 Posted October 13, 2004 where can we get the full length of amino acid sequences? i have very little bioinformatic background so please the more you explain the better it would be. thank you!
badchad Posted October 13, 2004 Posted October 13, 2004 Go to NCBI's website http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=Protein They have full nucleotide and protein sequences. In general, if an organism has been sequenced it's fairly easy to locate. The very difficult part is what to do once you have a sequence. There are many types of database matching programs which attempt to predict the function of a protein based on it's amino acid sequence. What exactly are you trying to do?
wonder Posted October 14, 2004 Author Posted October 14, 2004 thanks badchad! but the CDS is that the nucleotide sequence of the mature mRNA or premature mRNA? BWT i am a beginner and really want to learn and use the tools in bioinformatic. do you know any place or books which is simple but quite useful? thanks!
m/z Posted October 14, 2004 Posted October 14, 2004 NCBI is nice. But I also like the website http://www.expasy.org/ Beside the Swiss-Prot and TrEMBL protein databases, you can also find a lot of links to different other interesting sites, tools and documentations. The Swiss-Prot database has, to my opinion, a very nice data format. If a protein is well characterized, you can find the complete precursor sequence and easily get the information which part of the sequence belongs to the mature protein, sequence peptides and propeptides. Known mutations and PTMs are given as well. To get more detailed information, it is always good to have a look into the online-documentation of the databases you are using. In addition, there are some nice tutorials in the web, which are free. See for instance http://www.bioinformatik.de/cgi-bin/browse/Catalog/Research_and_Education/Online_Courses_and_Tutorials/ Regards, m/z
daisy Posted October 16, 2004 Posted October 16, 2004 You can get all the info m/z refers to on NCBI in my experience.
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