lukasbecker Posted September 5, 2011 Share Posted September 5, 2011 I am trying to see if the GFP protein is used with anti-bodies to label the proteins OR something else. It says GFP can be fused to any protein of interest using standard methods of recombinant DNA. I guess that means that an antibody isn't necessary but...what is a standard method of recombinant DNA? HELP! Link to comment Share on other sites More sharing options...
CharonY Posted September 5, 2011 Share Posted September 5, 2011 The fusion is done on the DNA level. I.e. you basically attach the gfp gene in-frame to your protein of interest, for example (details vary depending on what you want to get at the end). Link to comment Share on other sites More sharing options...
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