Chinmay1988 Posted September 7, 2011 Share Posted September 7, 2011 I was wondering if it is possible to run purified viruses in SDS PAGE gels? I am thinking of an experiment using hepatitis A virus and wanted to know its size in kDA. I do know that its ORF codes for a polyprotein which is 250 kDA in size, however if I load the virus onto the gel, will I get a similar size? Cheers Link to comment Share on other sites More sharing options...
CharonY Posted September 7, 2011 Share Posted September 7, 2011 (edited) Under non-denaturing conditions (i.e. not SDS) the capsid will not be of the same size as a the unprocessed polyprotein (electrophoretic mobility is quite different). Under denaturing conditions you will see the subunits. Edited September 7, 2011 by CharonY Link to comment Share on other sites More sharing options...
Chinmay1988 Posted September 8, 2011 Author Share Posted September 8, 2011 Thank you so much Link to comment Share on other sites More sharing options...
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