Housekeeping protein in denervated muscle

[Yossarian33]

Hi forum,

 

I am currently doing an undergrad thesis with a fellow undergraduate student (two separate projects)

 

We are both using denervated mouse muscle in our thesis. We are looking for a housekeeping (internal control) protein to probe for in our Western Blots. We have done test probes for alpha-tubulin but the literature indicates that tubulin may be upregulated following denervation, making this protein an unsuitable internal control.

 

Does anyone have experience with denervated muscle research and an idea of which housekeeping protein could be used; i.e. a protein who's expression is not altered by denervation?

 

We have been browsing the literature and have come up with many candidates but with no research outlining the expression of these proteins following denervation, we can't be sure they would be valid internal controls. We do know that alpha-tubulin would likely be a very bad internal control. (Our thesis coordinator has said that there is research both ways - that tubulin levels do not change following denervation or that they do change. We've only found articles with evidence of change.)

 

Thanks for any possible insight!