Dima Posted December 3, 2011 Share Posted December 3, 2011 ....separates proteins by size. But, technically does the charge affect the separation AT ALL or is negligible? I don't think anything should be negligible in science do you lol? Link to comment Share on other sites More sharing options...
whusean Posted December 3, 2011 Share Posted December 3, 2011 I wouldn't say charge is negligible. Granted, size of protein has a larger impact than charge, but its the mass/charge ratio which is taken into consideration. Link to comment Share on other sites More sharing options...
Dima Posted December 3, 2011 Author Share Posted December 3, 2011 (edited) yes, but the argument is that the SDS coats the proteins giving them all an equal charge supposedly. Is it true? Edited December 3, 2011 by Dima Link to comment Share on other sites More sharing options...
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