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Posted

....separates proteins by size. But, technically does the charge affect the separation AT ALL or is negligible? I don't think anything should be negligible in science do you lol?

Posted

I wouldn't say charge is negligible. Granted, size of protein has a larger impact than charge, but its the mass/charge ratio which is taken into consideration.

Posted (edited)

yes, but the argument is that the SDS coats the proteins giving them all an equal charge supposedly. Is it true?

Edited by Dima

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