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Labeling of Lipids (liposomes) using fluorescent dyes


Karapau

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Hi All

 

I would like to label some lipids that I have in order to produce labelled liposomes (I know that you can by Rhodamine-labeled lipid from Avanti, but I would like to use a different dye in my own lipids if possible). I cannot find any protocols in the literature or internet for this. My current lipids are: DOTAP, DSPC, PEG-CerC16 and Cholesterol.

 

Is there any procedure to incorporate FITC or TRITC in any of these lipids?

 

Thank you very much.

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