E.coli DH10B

[afsaneh]

Hi

 

DH10B is host of my plasmid , but we dont have DH10B in lab. can I use top10 or DH5a instead of DH10B? (base of my plasmid is pbluescript KS+ )

[CharonY]

IIRC it is a pBR322 derivative with nothing terribly fancy. As such Top and DH5alpha should both be fine. Main differences are found in the methylation/restriction systems (and recombination apparatus).