Another calibration question

[Gamewizard]

Hi, I know I have asked a few graph questions before but got another one that I am stuck on now

 

The concentration of a stock standard is 4mmol/L (Creatinine). This stock standard is diluted in water to give a set of working standards which are 0 mmol/L and 0.00ml, 0.1mmol/L and 0.125ml, 0.2 mmol/L and 0.25 ml, 0.5 mmol/L and 0.625, 0.8mmol/L and 1ml, 1.0mmol/L and 1.25 ml, and 2.0mmol/L with 2.5ml vol. (The mmol/L are concentrations and the ml are volumes of the standards)

 

The question says I have to plot this data as a calibration curve. I dont know which set of numbers i am supposed to plot and which is the X and Y axis.

 

Then I am supposed to determine the creatinine concentration in the samples (both urine and plasma) and the absorbances are :

 

Patient 1 : Plasma samples = 0.03 0.04 Urine samples: 0.44 0.45

 

Patient 2: Plasma samples = 0.125 0.13 Urine samples: 0.40 0.38

 

Please someone help me as this question is quite challenging !

 

 

Editing this as I found the corresponding absorbances as well for each standard !

Edited June 7, 2012 by Gamewizard

[jimmydasaint]

Hi, to start with, I assume I am correct in this assumption, you should plot a graph of the independent variable on the x-axis and the dependent variable (what you measured) on the y-axis. If you are clear on this point, how do you work out concentrations?

 

If I had a 0.25 mmol/L solution it contains 0.25 mmol per 1000 ml,

so it should have 0.25/1000 mmol per each ml. Has the penny droppped yet?