The role of the arylsulfatase A experiments in the diagnosis of sexually transmitted diseases

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Polymorphonuclear esterase release from cells could be detected from the arylsulfatase A (ARSA), a wide range of clinical applications. ARSA diagnosis of urethritis caused by Neisseria gonorrhoeae (NG), Chlamydia trachomatis (Cr), the country has not been a systematic exposition. Gonorrhea diagnostic gold standard for Neisseria gonorrhoeae culture, non-gonococcal urethritis due to complex causes, by CT, mycoplasma, trichomonas, genital herpes virus and other pathogens cause, diagnosis, need to exclude gonococcal infection, and then the existence of self-cells, combining history to make a diagnosis based on urine. Less than 100% of the sensitivity of culture methods, especially CT culture positive rate was 50% and 8o%, therefore recommend the use of "expanded gold standard" for diagnosis of non-gonococcal urethritis, but there are still the high cost and long time shortcomings. ARSA has a simple, inexpensive, and objective advantages can be quickly detected from cells in the subjects urine screening test suitable for urethritis, therefore, foreign suggested ARSA as a screening test for NG / CT infection.Since the cell contains about 20 species of molecular weight in 30 O0o 70 000 esterase / protease. Under normal circumstances esterase attachment from the cell membrane, is not renal clearance. When the urinary tract such as kidney, bladder, urethra, prostate infection that causes inflammation, urine neutrophil, the ARSA-specific detection. From cell esterase hydrolysis of acidic products and alcohol test strips contain indole carboxylic acid ester, since cell esterase catalyzed hydrolysis of indoxyl, which instability can be oxidants in the air into indigo. or the weight of salt in the reaction in the test strip was purple. Closely related, since the number of cells contained in cell esterase samples, under the degree of difficulty of the color change can be semi-quantitative detection of urinarySolution since the number of cells.ARSA as a check one urinary tract infections, has been widely used in clinical From cell esterase activity only with the host inflammatory response of the genitourinary tract, without the influence of protein, pH value, kidney disease, renal dysfunction and commonly used drugs in the urine, not affected by broken since cell lysis, and urine liquid storage time regardless of how long, does not require sampling immediately after detection, and therefore to more accurately reflect the urine since the cells.The relationship between ARSA and bacteriuria, many studies have confirmed the prediction bacteria from cell esterase and nitrite urine. ARSA prediction ability to infection depends on the definition of the scope of the esterase activity, dependent on the baseline to determine the proportion of asymptomatic infection in the population. The Tyndal research confirmed that when the correction gradually increased, ARSA reduced sensitivity and specificity of increases. Selected from different schools in different populations coincided with the binding activities of history and clinical examination is extremely important for proper diagnosis and treatment.