Chelex 100

[semeli]

hi all!

 

i want to use chelex 100 sodium form (Sigma 50-100 dry mesh) in order to remove calcium traces from my protein but i dont know how to use it.

 

any suggestion is always wellcome.

 

cheers,

s

[CharonY]

Check Dunn, M.F., et al., Biochemistry, vol. 19, 718 (1980). There are variation with differences in speed and recovery, but if you do not really intend to build a column or something similar the described approach should work fine. Just remember that the binding is highly pH dependent.

Edited July 1, 2012 by CharonY

[zazaayub]

hi..anyone know how to prepare 5 % chelex 100(sigma)?

[CharonY]

Normally w/v % is used. I.e. you just weigh in the desired amount of Chelex (say 5g) and then add ultrapure water (in this case 100 ml) to create a suspension with the given percentage.