Effect of temp on Savinase & Alcalase

[Guest Nyla]

Hi,

 

Im doing a A Level Project on the activity of these two different proteases when exposed to different temperatures, using photographic film as a detection method. I was going to do effect of pH but decided to do temp instead coz thot it wuld have more theory. Can someone please give me sum onfo about thse two enzymes (savinase and alcalase), structure of theirs etc to help me along...I'm goin to b usin a colourometer afta usin da photographic film..is der a gd method ne1 culd suggest? Seriously, any help at Allll appreciated please, dis is a reali importnt piece of c/w.

[m/z]

It seems that both, savinase and alcalase, are synonyms for subtilisin. Have a look at:

 

http://www.brenda.uni-koeln.de/index.php4