knochout of only one allele of gene-HOW?

[Kateryna1]

Hello!

 

I am not genetician, but more biochemist/cellular biologist. Therefore, my question is if it is possible and if yes,

then HOW it is possible to create a cell line with knochout of only one allele of gene?

I understand general principles of generating of full knockout. Don't jugde if the queastion is too naive.

I am grateful for an answer or a reference to source of wisdom.

[ecoli]

off the top of my head: this isn't a complete knockout, but if the variant is different enough, you could create a miRNA to knockdown only 1 allele.

[Kateryna1]

Thank you.

[Hobble]

Are the two alleles of the gene the same (ie. homozygous) or different (heterozygous)? If the latter, then miRNA could in fact work.