restriction enzyme

[daniton]

do eukayotic organisms made restriction enzyme ???

[CharonY]

None that are homologous to bacterial ones (AFAIK) or which are referred to as restriction enzymes. However, there enzymes with nuclease activities in eukaryotes. Often, they are involved in DNA repair. In addition there enzymes that are involved in DNA dynamics and replication with endonuclease activity. Generally they are not very sequence-specific however.