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Depends on what you really want to look at. The first step is to assess sequence similarities which is mostly done via alignments of the peptides under investigation. Clustal is commonly used as well as Dialign (among others).

 

The rest depends on the conclusions you want to draw, e.g. simply just sequence similarity, or true homology. For the latter (i.e. the identification of shared ancestry) just a comparison of two sequences is generally not enough, as you need reference sequences to really trace ancestry.

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