Immobilization of E. Coli

[Guest xelsslk]

Could anyone suggest a substrate that is easily available for me to bind E. coli cells onto 96 well plates.

 

I am using a recombinant protein from Bacteriophage Lambda to detect E. coli.

 

Thanks alot for any help given.

[ecoli]

E. coli have a natural affinity for plastic. Shouldn't they attach themselves to the plates, without any other substrate?

[Gilded]

"E. coli have a natural affinity for plastic. Shouldn't they attach themselves to the plates, without any other substrate?"

 

Let's not jump into rash conclusions here. For example, Michael Jackson seems to have a natural affinity for plastic too, yet he doesn't keep a plastic plate on his face (although I think he should).

[Guest xelsslk]

Hi there e. coli.

 

I would really be very interested about the protocol.

 

Would you mind passing me a simple one, maybe just right up here, on how to immobilize the cells, say from a cultured broth onto the 96 well plate?

 

Thanks alot for your help.

[ecoli]

I not sure that it would work after all. I was doing tissue cultures...that changes everything, doesn't it?

[OnlySinan]

"E. coli have a natural affinity for plastic. Shouldn't they attach themselves to the plates' date=' without any other substrate?"

 

Let's not jump into rash conclusions here. For example, Michael Jackson seems to have a natural affinity for plastic too, yet he doesn't keep a plastic plate on his face (although I think he should).[/quote']

 

HAHAH damn man, this is funny.