Co- culture experiment

[mohamed samir]

Dear ALl,

Alittle bit stricky for me to ask question like this.
Iam going to perform an experiment on a co- culture consists of both human A549 pneumocyte II cells and murine macrophage J774 cell line to test the effect of miRNAs on influenza virus replication .
I have no protocol for this and iam confused really whether I will be able to blend two cell line from different host. However, the media in both cells are differ , is this will pe appropriate medium for both cells ?
Should I used human macrophage cell line to be compatable with the A549 cell as it is from human ?
Dpepnding on which criteria, will our biculture succeed ?

I can summarize the question
Can I culture two differenrt cell line ( for example, A549 and J774 ) at the time both of them was cultured in different medium ?
Can I culture two differenrt cell line ( for example, A549 and J774 ) that isolated from two different animal host or human ?

Thanks

 

[Gaylord]

What do you hope to see?