Questions regarding gene splicing and it's techniques

[netangel999]

Hello, how are you?

 

Members, please assist me with the the following problems:

 

1. For performing gene splicing at home, i do acknowledge that need to have a restriction enzyme to cut apart my gene/DNA strand's base pairs/nucleotides such as: audonine, gaunine, cytocine, uracale and thymine, but can't i just use other types of equipment such as a pair of sissors? The sissors we use to cut papper? My biology teacher, back in high school stated that gene splicing can be performed by using sissors and applying heat on the gene we want to cut apart.

Is that true?

If yes, then why are we using restriction enzymes instead of the sissors we use at home? Why are we not using heat? I thought that heat causses the gene strand to open up. When the gene opens up, its very big about 30CM in size and we can edit them any time?

 

2. How do i edit the genes through light or light rays?

How do i add another base pair to the gene through light. So, first i apply heat on the gene to open it up then i add in the GENE nucleotides: actgu in the oder i want right? If it is not correct it will correct it self and nothing would go wrong?

 

 

3. I am trying to code myself a gene. I want this gene to perform the following action:

 

 

- When i consume fatty food, this gene will convert the fat into protien. I think this procedure of coding gene which turns fat into protiens, involves some over lapping technique.By this i mean that 2 genes are involved and one base pair of a gene is obverlapped with another base pair of another gene. So, as far as i know that there is no T= THYMINE INVOLVED, because that nucleotide actually causes a mutation in the muscles or the ginetic code. I know this is possable. Because, famous people such as bands, movie actors/actressses and movie stars usuualy take it.

 

 

- Please do NOT STATE ANYTHING LIKE THIS: THERE IS NO SUCH THING your body must get protein and then burn off fat. You cannot convert it into protien. I am 100 percent sure that there is a gene code without the use of THYMINE base pair that can have the results that i ask above. Be carfule and give me links to all websites you guys refered to.

 

Can anybody please aid me code this gene? I know that this gene uses the following base pairs exept for T.

 

:::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::::;

AUDONINE, GAUNING, CYTOZINE AND URACLE WHICH IS FOUND IN RNA not DNA.

 

Thanks in advance.

Take care

Netnagel999

[CharonY]

Oh dear, sorry to say, but there is quite a bit of misinformation. First regards to gene splicing, in biology splicing refers mostly to RNA maturation. What is being described, most people would refer to as a cloning experiment (e.g. cloning a DNA fragment into another). I am aware that some people do refer to it as gene splicing, but I found that to be very rare in scientific literature (and usually in very specific contexts).

 

As for the rest:

Please, the bases are adenine, guanine, cytosine and thymine (or uracil).

1) heat first opens up the double strand. To break the DNA sugar backbone you need to do something else. Restriction enzymes are used because they are one of the few techniques that actually allow precise sequence recognition. Using scissors is beyond ridiculous. Ten base pairs mount to roughly 2 nanometers, Good luck in finding those (never mind the impossible task of discerning the bases).

2) editing through light rays? Uh you could try to introduce UV mutations, I guess...

3) Claiming that something is possible does not make it so. Or even likely. Nor even slightly plausible. Also note that uracil merely replaces thymine. For what it is worth...

[netangel999]

hey thanks for the reply.

 

regarding the ULTRA VIOLET light, what do you mean by "introducing mutations"?

 

I want to use UV because i thought it would enlarge the gene size into 100CM or so. I have heard people do that.

 

 

Why are you telling me that papper cutting sissors are rediculous?

 

These siccors do the job i think. if i am wrong please do let me know. My teacher in school once told me that i can use PAPER sissors instead of the biological ones(bacteria forms) to cut DNA/GENE nucleotides or BASE pairs. What if i want to edit a gene in my finger. Supposing i want tp make my middle finger LONGER how do i do that?

 

 

Can you please list the codons?

 

 

 

 

In this website there is a online activity for students to edit genes:

 

http://www.biotechnologyonline.gov.au/popups/int_splicing.html

 

 

 

ok now this website has 4 colors: blue , orange , red and green

 

 

In the website, the blue color means, Audanine, the orange one is THYMINE, the red one is cyosine and the green one is guanine.

 

So IN REAL LIFE WOULD I EXPECT THE COLOR OF THE BASE PAIRS the same on the website?

 

If i were to find the codon for THYMINE. how would i find it in the DNA / gene STRAND???????

does the gene codons have colors on them?

 

 

Thanks ALOT.

 

 

!!!!

I SOMEWHAT UNDERSTAND. WE PERFORMED THIS IN HIGH SCHOOL BUT I HAVE LOST MY NOTES.

netangel999.

PS....

 

 

Dear, CharonY.

I want to extend the legnth of my middle finger. Just for fun AND THEN REDO IT.
Can YOU PLEASE TELL ME HOW TO DO THATA WITHOUT THE USE OF A MICROSCOPE OR BIOlogical sissors/bacteria?

I want to use my papper cutting sissors instead as my biology teacher stated in class and unwrap the gene in ultraviolet light or ANOTHER HEAT source.

I want to know the CODONS from you since you are an expert. You have a high rand in this science forum and you should be able to answere most of the question

I also want to know about the gene codons to extend my hieght or i want to become more taller.

People have told me that genes are located everywere inside our body but there must be in a specific location. For examople, for hieght there must be one near or in the back bone?

AM I RIGHT?

 

The gene for the back bone has to exsist in a nerve or something?

 

So just tell me are everyone differant as in does everyone HAVE differant place were the genes are kept in the backbone or are they still in the same place????

THANKS

netangel...

[Ringer]

hey thanks for the reply.

 

regarding the ULTRA VIOLET light, what do you mean by "introducing mutations"?

He means that UV light is a mutagen and could cause mutations of the base pairs.

 

I want to use UV because i thought it would enlarge the gene size into 100CM or so. I have heard people do that.

Even if this did stretch the gene (I have never heard of this)the bonds would break before you even got them to 1 mm, let alone a meter.

 

Why are you telling me that papper cutting sissors are rediculous?

The scissors are many orders of magnitude larger than what you are trying to cut and not nearly precise enough. It's like saying you are going to use a couple of sticks of TNT to cut a single hair off of a baby.

 

These siccors do the job i think. if i am wrong please do let me know. My teacher in school once told me that i can use PAPER sissors instead of the biological ones(bacteria forms) to cut DNA/GENE nucleotides or BASE pairs. What if i want to edit a gene in my finger. Supposing i want tp make my middle finger LONGER how do i do that?

The teacher was wrong. You could theoretically introduce a mutation in your finger to make it grow, but there is no way you would succeed practically. You would have to mutate every cell in your skin, muscle, tendon, bone, etc. And since much of growth is determined by nutrition you wouldn't know if the mutation would even do anything.

 

 

Can you please list the codons?

Adenine, Thymine, Cytosine, Guanine for DNA

Adenine, Uracil, Cytosine, Guanine for RNA

So IN REAL LIFE WOULD I EXPECT THE COLOR OF THE BASE PAIRS the same on the website?

No, it would be too small for you to hope to see.

 

If i were to find the codon for THYMINE. how would i find it in the DNA / gene STRAND???????

does the gene codons have colors on them?

Use a computer program or restriction agents for whatever base pattern you are looking for.

 

[CharonY]

What Ringer said. What you propose simply does not work. And it would require a fundamental lecture in basic genetics to make you understand why it is so.

For instance, the DNA composition is identical in each of our cells, there is not difference regardless of position. Simplified, genes mostly work by coding for proteins, and those (mostly) biological actors. The key aspect is that expression is finely tuned by regulatory mechanisms that result in certain biological actions such as cell differentiation. But really, I would suggest that you at least check the wikipedia pages for DNA and some genetics and then read up in a text book, if you are genuinely interested.

 

There is no way to impart that amount of info within a short post.