BRCA1-primers design

[manu26]

Hello every one,

 

I am designing primers for BRCA1 gene. I have two questions:

 

1. How can I find the stop codon for this gene.

2. what are the instruction I should be attention to when designing primers for this gene?

 

Thank you for you help

[CharonY]

1) If you look at database entry you will generally see the complete ORF. Depending on what you need (e.g. if you also want up- and downstream sequences) you may have to switch viewers that provide genomic views and you may need to search a little bit (using the annotated genome sequence).

 

2) There a hundreds of websites discussing primer design. Much can depend on the type of fragment you want to amplify (e.g. length, extensions, tags etc) as well as the polymerase. But for a general primer on that topic I would just google it (e.g. http://www.premierbiosoft.com/tech_notes/PCR_Primer_Design.html) and see what applies to you.